Benassi L, Ottani D, Fantini F, Marconi A, Chiodino C, Giannetti A, Pincelli C (1997) 1, 25-dihydroxyvitamin D3, transforming growth factor beta 1, calcium, and ultraviolet B radiation induce apoptosis in cultured human keratinocytes. Keratinocytes are the epidermal cells that produce keratin. Langley RG, Walsh N, Nevill T, Thomas L, Rowden G (1996) Apoptosis is the mode of keratinocyte death in cutaneous graft-versus-host disease. lnachi S, Mizutani H, Shimizu M (1997) Epidermal apoptotic cell death in erythema multiforme and Stevens-Johnson syndrome. Cytokines are polypeptide growth factors produced by most nucleated cells in the body, including epithelial cells, keratinocytes, and Langerhans cells in the skin. Yoo YH, Gilliam AC, Whitaker-Menezes D, Korngold R, Murphy GF (1997) Experimental induction and ultrastructural characterization of apoptosis in murine acute cutaneous graft-versus-host disease. Cite as. Koh JY, Gwag BJ, Lobner D, Choi DW (1995) Potentiated necrosis of cultured cortical neurons by neurotrophins. This is a preview of subscription content. Shiohara T, Moriya N, Tanaka Y, Arai Y, Hayakawa J, Chiba M, Nagashima M (1988) Immunopathologic study of lichenoid skin diseases: correlation between HLA-DR-positive keratinocytes or Langerhans cells and epidermotropic T cells. The HF mesenchyme is usually recreated with specialized dermal papilla (DP) cells, whereas the epithelial fraction is commonly reconstructed using ker- atinocytes (KCs) isolated from different follicular sources, including the bulge [5], the outer root sheath (ORS) [6–10], or the hair bulb [8, 11]. Casciola-Rosen LA, Anhalt G, Rosen A (1994) Autoantigens targeted in systemic lupus erythematosus are clustered in two populations of surface structures on apoptotic keratinocytes [see comments]. Eukaryotic cells express germ line-encoded receptors of the innate immune system, pathogen recognition receptors (PRRs), that recognize invariant molecular motifs known as pathogen-associated molecular patterns (PAMPs) ( 46 ). Chin L, Schreiber-Agus N, Pellicer I, Chen K, Lee HW, Dudast M, Cordon-Cardo C, DePinho RA (1995) Contrasting roles for Myc and Mad proteins in cellular growth and differentiation. Reynolds NJ, Talwar HS, Baldassare JJ, Henderson PA, Elder JT, Voorhees JJ, Fisher GJ (1993) Differential induction of phosphatidylcholine hydrolysis, diacylglycerol formation and protein kinase C activation by epidermal growth factor and transforming growth factor-alpha in normal human skin fibroblasts and keratinocytes [published erratum appears in Biochem J 1993 Nov 1; 295 (pt 3): 903]. Wright SC, Kumar P, Tam AW, Shen N, Varma M, Larrick JW (1992) Apoptosis and DNA fragmentation precede TNF-induced cytolysis in U937 cells. In stem cell: Epithelial stem cells. pp 121-147 | These primary cell types have potential commercial value in a wide range of … Allsopp TE, Wyatt S, Paterson HF, Davies AM (1993) The proto-oncogene bd-2 can selectively rescue neurotrophic factor-dependent neurons from apoptosis. Hebert JM, Rosenquist T, Gotz J, Martin GR (1994) FGFS as a regulator of the hair growth cycle: evidence from targeted and spontaneous mutations. Keratinocytes are the most common skin cells. Stoll SW, Benedict M, Mitra R, Hiniker A, Elder JT, Nunez G (1998) EGF receptor signaling inhibits keratinocyte apoptosis: evidence for mediation by bcl-xL. Norris DA (1993) Pathomechanisms of photosensitive lupus erythematosus. They make keratin, a protein that provides strength to skin, hair, and nails. Mason IJ (1994) The ins and outs of fibroblast growth factors. Eckert RL, Crish JF, Robinson NA (1997) The epidermal keratinocyte as a model for the study of gene regulation and cell differentiation. The epidermis is made up of stratified epithelium. Obeid LM, Linardic CM, Karolak LA, Hannun YA (1995) Programmed cell death induced by ceramide. Werner S, Breeden M, Hubner G, Greenhalgh DG, Longaker MT (1994) Induction of keratinocyte growth factor expression is reduced and delayed during wound healing in the genetically diabetic mouse. Godar DE, Lucas AD (1995) Spectral dependence of UV-induced immediate and delayed apoptosis: the role of membrane and DNA damage. differential regulation of mc1–1 and bc1–2 protein production suggests a unique role for mcl-1 in control of programmed cell death. Taken together, these results demonstrate that epithelial lineage cell such as keratinocytes proliferate at a similar rate on nanopatterns relative to flat surfaces but mesenchymal lineage cell such as fibroblasts exhibit reduced proliferation. For this technique, primary (P0) cultures were fed with approximately 30–35 ml of Epilife® low calcium medium plus EDGS supplement in contrast to the standard volume of 15 ml/T-75 flask. Watt FM, Kubler MD, Hotchin NA, Nicholson LJ, Adams JC (1993) Regulation of keratinocyte terminal differentiation by integrin-extracellular matrix interactions. Middleton MH, Norris DA (1995) Cytokine-induced ICAM-1 expression in human keratinocytes is highly variable in keratinocyte strains from different donors. Keratinocytes form in the deep, basal cell layer of the skin and gradually migrate upward, becoming squamous cells before reaching the … Norris DA (1995) Editorial: Differential control of cell death in the skin. Furukawa F, Kashihara Sawami M, Lyons MB, Norris DA (1990) Binding of antibodies to the extractable nuclear antigens SS-A/Ro and SS-B/La is induced on the surface of human keratinocytes by ultraviolet light (UVL): implications for the pathogenesis of photosensitive cutaneous lupus. Role of Ia+ keratinocytes in the epidermotropic migration of the T cells. Chrysomali E, Lozada-Nur F, Dekker NP, Papanicolaou SI, Regezi JA (1997) Apoptosis in oral erythema multiforme. © 2020 Springer Nature Switzerland AG. Zhang Z, Vuori K, Reed JC, Ruoslahti E (1995) The alpha 5 beta 1 integrin supports survival of cells on fibronectin and up-regulates bc1–2 expression. Under basal conditions, they require about 2 weeks to exit the nucleated compartment and an additional 2 weeks to move … Chronic inflammatory skin diseases are thought to result from the interaction of immune and/or inflammatory cells and resident epithelial cells. Part of Springer Nature. Barreca A, De Luca M, Del Monte P, Bondanza S, Damonte G, Cariola G, Di Marco E, Giordano G, Cancedda R, Minuto F (1992). J. Pena JC, Fuchs E, Thompson CB (1997) Bel-x expression influences keratinocyte cell survival but not terminal differentiation. Therefore, these cells are sometimes called the basal cells or basal keratinocytes. Miyashita T, Krajewski S, Krajewska M, Wang HG, Lin HK, Liebermann DA, Hoffman B, Reed JC (1994) Tumor suppressor p53 is a regulator of bc1–2 and bax gene expression. They are also present in epithelial cells in general. Young JDE, Podack ER, Cohn ZA (1986) Properties of a purified pore-forming protein (Perforin 1) isolated from H-2 restricted T cell granules. The stratum is the outermost layer that contains dead keratinocyte cells. Keratinocytes can respond to cell injury and cell stress and can sense pathogens, thus mediating immune responses . Keratinocytes and cytokine/growth factors. Gniadecki R, Hansen M, Wulf HC (1997) Two pathways for induction of apoptosis by ultraviolet radiation in cultured human keratinocytes. Growing evidence suggests that cells other than fibroblasts (e.g., keratinocytes, mast cells, and macrophages) actively participate in scar pathogenesis . Keratin filaments are abundant in keratinocytes in the cornified layer of the epidermis; these are proteins which have undergone keratinization. The keratinocytes slowly move outward through the epidermis as they mature, and they eventually die and are…. The HF mesenchyme is usually recreated with specialized dermal papilla (DP) cells, whereas the epithelial fraction is commonly reconstructed using keratinocytes (KCs) isolated from different follicular sources, including the bulge [ 5 ], the outer root sheath (ORS) [ … Materials and methods 2.1. Exp. The principal functions of keratinocytes are to provide an intact epithelial covering for the body and an impermeable barrier resisting loss of water, minerals and protein, and preventing the entrance of toxic environmental agents. Keratinocytes originate in the basal layer from the division of keratinocyte stem The keratinocytes slowly move outward through the epidermis as they mature, and they eventually die and are…. Stalder T, Hahn S, Erb P(1994) Fas antigen is the major target molecule for CD4. These ectoderm-derived cells are squamous and originate in the bottommost stem cell pools of the stratum basale. Keratinocytes are the epithelial cells which comprise the epidermis of the skin and the epithelium of the oral mucous membranes. In terminal differentiation keratinocytes form the stratum corneum, composed of dead keratinocytes termed corneocytes and a complex phospholipid permeability barrier. Johnson R, Staiano-Coico L, Austin L, Cardinale I, Nabeya-Tsukifuji R, Krueger JG (1996) PUVA treatment selectively induces a cell cycle block and subsequent apoptosis in human T-lymphocytes. The identification of p63 as a keratinocyte stem cell marker will be of practical importance for the clinical application of epithelial cultures in cell therapy as well as for studies on epithelial tumorigenesis. Johnson NL, Gardner AM, Diener KM, Lange-Carter CA, Gleavy J, Jarpe MB, Minden A, Karin M, Zon LI, Johnson GL (1996) Signal transduction pathways regulated by mitogen-activated/extracellular response kinase kinase kinase induce cell death. Feliciani C(1), Gupta AK, Sauder DN. Hockenbery DM, Zutter M, Hickey W, Nahm M, Korsmeyer SJ (1991) Bcl-2 protein is topographically restricted in tissues characterized by apoptotic cell death. Suda T, Takahashi T, Golstein P, Nagata S (1993) Molecular cloning and expression of the Fas ligand, a novel member of the tumor necrosis factor family. Krajewski S, Bodrug S, Krajewska M, Shabaik A, Gascoyne R, Berean K, Reed JC (1995) Immunohistochemical analysis of mc1–1 protein in human tissues. Typically, 95% of the cells in the epidermis are keratinocytes. Keratinocytes are the most common type of skin cells. Ben-Bassat H, Rosenbaum-Mitrani S, Hartzstark Z, Shlomai Z, Kleinberger-Doron N, Gazit A, Plowman G, Levitzki R, Tsvieli R, Levitzki A (1997) Inhibitors of epidermal growth factor receptor kinase and of cyclin-dependent kinase. Werner S, Smola H, Liao X, Longaker MT, Krieg T, Hofschneider PH, Williams LT (1994) The function of KGF in morphogenesis of epithelium and reepithelialization of wounds. Ahmad W, Faiyaz ul Haque M, Brancolini V, Tsou HC, ul Haque S, Lam H, Aita VM, Owen J, deBlaquiere M, Frank J, Cserhalmi-Friedman PB, Leask A, McGrath JA, Peacocke M, Ahmad M, Ott J, Christiano AM (1998) Alopecia universalis associated with a mutation in the human hairless gene. Packham G, Cleveland JL (1995) C-myc and apoptosis. Epidermal keratinocytes (skin cells) are highly specialized epithelial cells designed to perform a very specific function: to form a barrier to separate and protect the organism from its environment. Henseleit U, Rosenbach T, Kolde G (1996) Induction of apoptosis in human HaCaT keratinocytes. Norris DA (1989) Immunological cytotoxicity of cutaneous cellular targets. Poumay Y, Pittelkow MR (1995) Cell density and culture factors regulate keratinocyte commitment to differentiation and expression of suprabasal K1/K10 keratins. J. Rosette C, Karin M (1996) Ultraviolet light and osmotic stress: activation of the JNK cascade through multiple growth factor and cytokine receptors. Jetten AM (1980) Retinoids specifically enhance the number of epidermal growth factor receptors. Meredith JE, Jr., Fazeli B, Schwartz MA (1993) The extracellular matrix as a cell survival factor. Keratinocyte culture is also used to investigate various cellular and molecular mechanisms involved in different skin pathologies. Mammalian epidermis ) actively participate in scar pathogenesis by neurotrophins keratinocytes is highly variable in keratinocyte strains from different.! 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